A Case-Control study for Genetic Susceptibility Genes of Type 2 Diabetes with History of Obesity in Jordanian Population of Arab Descent

Abstract

The aim of this study is to detect and characterize genetic polymorphisms that may influence susceptibility to type 2 diabetes (T2D) patients with history of obesity in Jordanian population.  We designed a case-control study involving 600 Jordanian individuals (300 type 2 diabetes cases with history of obesity and 300 healthy individuals with no history of diabetes and obesity). Single Nucleotide Polymorphisms (SNPs) within candidate genes (ENPP1 (rs7754561), FTO (rs9939609), MC4R (rs1211913564, rs17782313), and NPC1 (rs1805081, rs1788799)) were genotyped using a Sequenom MassARRAY system (iPLEX GOLD). The only SNP associated with T2D with history of obesity in this study was rs7754561 in the A/G 3’UTR region of ENPP1 gene, with overall estimate of p-value = 0.025 (χ2 (2, N = 600) = 9.33).  It has been reported that ENPP1, Ectonucleotide Pyrophosphatase/Phosphodiesterase 1, plays a major part in facilitating insulin resistance and in the progression of both diabetes and obesity, signifying that a fundamental molecular genetic mechanism is common to both diseases in Jordanian population.