Role of Oleuropein on Drosophila Induced ZnOTiO2 Nanocomposite

Abstract

Today, in parallel with the development of nanotechnology, the increase in the concentration of the nanoparticles in the nature has gained importance and daily the risks and effects of nanoparticles on the environment and human health are increasing. In this study, the possible toxic effects of increasing usage and applications of nanocomposite ZnOTiO2 on Drosophila melanogaster larvae and the protective role of Oleuropein (OLE) in order to eliminate this effect have been investigated. For this purpose, two separate sets of experiment were prepared, one a control group including ZnOTiO2 with distilled water and OLE and another ZnOTiO2+OLE application group. In order to determine the oxidative parameters in mature flies, total oxidant status (TOS), total antioxidant capacity (TAC) and oxidative stress index (OSI) were measured in mature flies subjected ZnOTiO2 and ZnOTiO2+OLE in larvae state. In ZnOTiO2 applications groups the level of TOS was relatively high and TAC was low compared to control group; in ZnOTiO2+OLE group while TOS was decreasing and TAC was increasing in compared with the only ZnOTiO2 application group. The observed TAC and TOS changes compared to control group values were statistically significant (p<0.05). These toxic effects of ZnOTiO2 nanocomposite observed in Drosophila is being provided through the increasing the formation of reactive oxygen species, leading to oxidative stress and lipid peroxidation. OLE is believed to play a protective role against the harmful effects of ZnOTiO2 nanocomposites thanks to OLE’s antioxidant role by preventing the formation of free radicals, inhibiting lipid peroxidation and stimulating the detoxification enzymes.